Most studies have aimed to explore the carcinogenicity of this o-anisidine. Rats Treated with BBN (N-Butyl-N-(4-Hydroxybutyl)nitrosamine) Lose Body Weight, Bladder Histology Shows Incidence of Papillary and Nodular Hyperplasia, Papillomas, and Bladder Cancer A significant increase. Similar experiments on mice showed the induction of bladder papillomas and carcinomas.

This was revealed when ALKBH8 transgenic mice displayed increased bladder tumor mass and invasiveness in an N-butyl-N-(4-hydroxybutyl)-nitrosamine-induced bladder cancer model, indicating high levels of ALKBH8 Expression is important for bladder cancer growth and progression [43]. Therefore, ALKBH8 may be a drug target in bladder cancer.

Therefore, dietary vitamin C inhibits N-butyl-N-(4-hydroxybutyl)-nitrosamine, N-bis(2-hydroxypropyl)-nitrosamine and N-ethyl-N- Induction of hepatic tumors in rats by hydroxyethylnitrosamine. 77 Administration of vitamin C also inhibited the development of liver tumors induced by diethylnitrosamine78 or diethylnitrosamine/2-acetamidofluorene.

Therefore, dietary vitamin C does not affect liver tumors induced by nitrosamines,82 aflatoxin,83 diethylnitrosamine,84 N-butyl-N-(4-hydroxybutyl)nitrosamine.